Figure 3
RT-qPCR analysis of microRNAs differentially expressed in DGF. MicroRNAs identified as differentially expressed in TLDA profiling (Fig. 2) were quantified by RT-qPCR in ‘first-pass’ urine in 33 patients, whose donor and recipient characteristics are described in Table 1. MicroRNAs were quantified by Taqman individual microRNA assay, and data are normalised to miR-Cel-39 before calculation of relative expression by the delta delta Ct method42. 6 of 8 target microRNAs were up regulated in CD-DGF. Data are plotted as mean ± SEM. Number of patients in each group: LD-No DGF (n = 10); CD-No DGF (n = 10); and CD-DGF (n = 13). Statistical significance: *p < 0.05, **p < 0.01.
