Figure 6
RT-qPCR analysis of microRNAs differentially expressed in DGF in a second validation cohort from a different transplant centre. Urine samples were collected in the first week post-transplantation from 50 kidney transplant recipients at Newcastle transplant centre and grouped into ‘DGF’ (n = 13) and ‘No DGF’ (n = 37). Following RNA extraction, RT-qPCR was performed to quantify the 6 validated microRNAs (miR-9, -10a, -21, -29a, -221, and -429) using Taqman individual microRNA assays. Data was normalised to miR-191 before calculation of relative expression by the delta delta Ct method42. Data are plotted as mean ± SEM.
