Figure 3

PTI-00703 cat’s claw was also a potent disaggregator/disrupter of Aβ 1–42 fibrils almost instantly. (a) PTI-00703 cats claw caused a dose-dependent disruption/disaggregation of Aβ 1–42 fibrils as assessed by Thioflavin T fluorometry. A dose dependent disaggregation/disruption of Aβ 1–42 fibrils was observed nearly immediately (upon mixing). **p < 0.05, ***p < 0.001 by Student’s t-test. Bars represent mean +/− SEM. n = 5. (b) Congo red staining of Aβ 1–42 fibrils by day 0 demonstrated a robust red/green birefringence under polarized light indicative of amyloid fibrils. Scale bar = 25 µm. (c) Aβ1–42 in the presence of PTI-00703 cat’s claw near completely diminished Congo red staining indicative of a disruption/disaggregation of Aβ 1–42 fibrils (Aβ: PTI-00703 cat’s claw 1:1 wt/wt). Scale bar = 25 µm. (d) Aβ 1–42 by day 0 also showed positive Thioflavin S fluorescence under fluorescent light. Scale bar = 25 µm. (e) PTI-00703 cat’s claw disaggregated/disrupted Aβ 1–42 fibrils as shown by a marked reduction in Thioflavin S fluorescence (Aβ: PTI-00703 cat’s claw 1:1 wt/wt). Scale bar = 25 µm. (f) Electron microscopy at day 0 demonstrated abundant Aβ 1–42 amyloid fibrils with a characteristic fibril diameter of 10-20 nm. Scale bar = 100 nm (g) Aβ 1–42 fibrils in the presence of PTI-00703 cat’s claw demonstrated primarily the formation of amorphous non-fibrillar material, nearly immediately upon mixing (Aβ: PTI-00703 cat’s claw 1:1 wt/wt). Scale bar = 100 nm.