Figure 9 | Scientific Reports

Figure 9

From: The Amazon rain forest plant Uncaria tomentosa (cat’s claw) and its specific proanthocyanidin constituents are potent inhibitors and reducers of both brain plaques and tangles

Figure 9

3H-PTI-777 crossed the blood-brain-barrier and entered the brain parenchyma within 2 minutes following peripheral administration. 0.9 mCi of 3H-PTI-777 with specific activity of 1.25 mCi/mg (at a concentration of 1 mCi/ml) was prepared and used for animal studies. (a) Figure demonstrates the HPLC profile of unlabeled PTI-777 which is nearly identical to the HPLC profile of (b) 3H-PTI-777. Fractions g, f, h, i, j, k and m/n are shown. (c) The distribution of radioactivity measured in 0.5 ml fractions that was eluted from the HPLC column and collected beginning at 16.5 minutes. This graph demonstrates that the HPLC peaks of PTI-777 also coincide with the greatest radioactivity. (d) Penetration of 3H-PTI-777 into rat brain, with radioactivity present in brain tissue in 2 minutes and lasted over a 24-hour period. n = 14 (7 females and 7 male Sprague-Dawley rats) (e) Penetration of 3H-PTI-777 into mouse brain tissue (CD-1 mice) within 2 minutes after being in the blood. n = 16. Bars represent mean +/− SEM.

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