Figure 3
From: Widespread PERK-dependent repression of ER targets in response to ER stress
ER stress gene expression programs are recapitulated in NIH3T3 cells. (A) Polysome profiling of NIH3T3 cells showed major repression following 2 h of Tg treatment, and a partial relaxation at 7 h post Tg treatment (P/M ratios are: Control – 1.7, Tg 2 h – 0.42, Tg 7 h – 0.65). (B–D) Log2 fold changes (LFCs) of the genes from the three major ER stress gene expression programs, namely early induction (B), late induction (C) and repression (D), were calculated using ribosome footprint profiling data of Tg treated NIH3T3 cells for either 2 h (red) or 7 h (green). CDF plots demonstrate the cumulative fraction of each set of genes (y-axis), as a function of their log2 fold change (LFC) (x-axis) relative to control. Background distributions (LFC values of all expressed genes) in either 2 h Tg or 7 h Tg are marked in dashed grey lines. The analysis demonstrated that the three ER stress gene expression programs that were identified in MEFs were recapitulated in NIH3T3 cells. T-test p-values between each distribution and its respective background are indicated.
