Table 3 PCR reaction mixture.
Components | Add volume (μL) |
|---|---|
ddH2O | 15 |
10 × Ex buffer (Mg2+ plus) | 2 |
dNTP (2.5 mM each) | 1.6 |
AGRP52 (10 μM) | 0.5 |
AGRP53 (10 μM) | 0.5 |
Template (gDNA) (50 ng/μL) | 0.3 |
Ex Taq (5 U/μL) | 0.1 |
Total volume | 20 |
Components | Add volume (μL) |
|---|---|
ddH2O | 15 |
10 × Ex buffer (Mg2+ plus) | 2 |
dNTP (2.5 mM each) | 1.6 |
AGRP52 (10 μM) | 0.5 |
AGRP53 (10 μM) | 0.5 |
Template (gDNA) (50 ng/μL) | 0.3 |
Ex Taq (5 U/μL) | 0.1 |
Total volume | 20 |
