Table 1 Properties of concentration distribution and size distribution of RBC samples obtained from the CBC of whole blood and used as additional information in the optimisation (left) in comparison to the optimisation results (right). MCHC = mean(cHb), HDW = std(cHb)/mean(cHb), \({\rm{MCV}}={\rm{mean}}(V)=\frac{4\pi }{3}\,{\rm{mean}}({R}^{3})\), RDW = std(V)/mean(V), where std denotes the standard deviation. cRBC is the RBC concentration given for the fresh whole blood sample and the washed RBCs and ϕ the dilution factor applied for the extinction measurement of the washed RBCs. The numbers in parentheses are the estimated standard uncertainties, referred to the last digit.
Volunteer | CBC | ϕ | Optimisation Result | ||||||
|---|---|---|---|---|---|---|---|---|---|
cRBC/pL−1 | \(\frac{{\bf{MCHC}}}{{\bf{g}}{{\bf{L}}}^{-{\bf{1}}}}\) | \(\frac{{\bf{MCV}}}{{\bf{fL}}}\) | \(\frac{{\bf{RDW}}}{{\boldsymbol{ \% }}}\) | \(\frac{{\bf{HDW}}}{{\boldsymbol{ \% }}}\) | \(\frac{{\bf{MCV}}}{{\bf{fL}}}\) | \(\frac{{\bf{RDW}}}{{\boldsymbol{ \% }}}\) | |||
fresh | washed | ||||||||
A | 4.62 | 4.58 | 329 (6) | 86.0 (1.0) | 12.7 (1.0) | 1670 | 3.7(4) | 85.7(1.0) | 12.7 (1.0) |
B | 6.55 | 4.02 | 324 (10) | 63.0 (2.2) | 15.7 (1.2) | 1430 | 6.4(3) | 66.0(2.2) | 15.8(1.2) |
C | 4.42 | 4.47 | 331 (6) | 81.5 (1.0) | 15.2 (1.0) | 3240 | 4.6(5) | 81.5(1.0) | 15.2(1.0) |
D | 4.04 | 4.48 | 352 (6) | 99.5 (1.0) | 12.5 (1.0) | 3240 | 4.6(3) | 98.2(0.9) | 12.5(1.0) |
E | 4.28 | 5.19 | 336 (6) | 91.3 (1.0) | 12.3 (1.0) | 3240 | 3.5(5) | 91.1(1.0) | 12.3(1.0) |
F | 4.80 | 4.05 | 352 (6) | 89.5 (1.0) | 12.1 (1.0) | 1620 | 4.2(3) | 86.5(1.0) | 12.0(1.0) |
