Table 2 Phenotypic properties distinguishing the isolates from one another and from their closest phylogenetic neighbours.
Isolate LB4 | Isolate LB41 | M. chalcea DSM 43026T | Isolate LB19 | M. ureilytica GUI23T* | Isolate LB32T | M. saelicesensis Lupac 09T | Isolate LB39T | M. chokoriensis JCM 13248T | |
|---|---|---|---|---|---|---|---|---|---|
Biochemical tests: | |||||||||
Catalase | + | + | − | + | + | + | + | + | + |
Oxidase | − | − | − | − | + | − | + | + | + |
API ZYM tests: | |||||||||
Acid phosphatase | + | + | + | + | − | − | + | + | + |
Alkaline phosphatase | + | + | + | − | + | − | − | + | + |
α-Chymotrypsin | + | + | + | + | + | − | + | + | − |
Cystine arylamidase | + | + | − | + | + | + | + | + | + |
α-Galactosidase | + | + | + | + | − | + | + | + | + |
N-acetyl-β-Glucosaminidase | − | − | + | + | + | + | + | + | − |
α-Glucosidase | + | + | + | + | − | + | + | + | + |
β-Glucosidase | + | + | + | + | − | + | + | + | + |
β-Glucuronidase | + | + | + | + | − | + | − | + | + |
α-Fucosidase | − | − | − | − | − | − | + | − | − |
Lipase (C14) | + | + | + | + | − | + | + | + | + |
Leucine arylamidase | + | + | − | + | + | + | + | + | + |
α-Mannosidase | − | − | − | + | − | − | + | + | − |
Valine arylamidase | + | + | − | + | + | + | + | + | + |
GENIII BIOLOG microplate tests: | |||||||||
(a) Oxidation of amino acids: | |||||||||
L-Alanine | + | − | − | − | − | + | + | + | + |
L-Arginine | + | + | + | − | − | + | − | + | − |
D-Aspartic acid | + | + | + | − | − | − | − | − | − |
Glycyl-L-proline | − | − | − | − | − | − | + | − | + |
L-Histidine | − | − | + | − | − | − | − | − | − |
D-Serine #2 | − | − | − | − | − | − | − | + | − |
L-Serine | − | − | − | − | − | + | − | + | + |
(b) Oxidation of sugars: | |||||||||
D-Arabitol | − | − | − | + | + | − | − | − | − |
D-Fucose | − | − | + | − | − | − | − | − | − |
L-Fucose | − | + | + | + | + | + | + | + | + |
3-O-methyl-D-Glucose | − | + | + | + | + | + | + | + | + |
N-acetyl-D-Galactosamine | + | − | + | + | + | + | + | + | + |
Glucuronamide | − | − | − | − | − | − | − | − | + |
Glycerol | − | + | + | − | − | + | − | + | + |
myo-Inositol | − | − | − | − | − | + | + | − | − |
α-D-Lactose | + | + | + | − | − | − | − | + | + |
D-Mannitol | − | − | − | + | + | + | − | − | + |
L-Rhamnose | − | − | + | − | − | + | + | + | + |
D-Salicin | − | + | + | + | + | + | + | + | + |
D-Sorbitol | − | − | + | + | + | + | + | − | + |
(c) Oxidation of organic acids: | |||||||||
Bromo-Succinic acid | − | − | − | + | + | + | + | + | − |
Butyric acid | + | + | + | − | − | − | − | + | − |
β-hydroxy-Butyric acid | + | + | − | + | + | + | + | − | + |
γ-amino-n-Butyric acid | − | − | + | − | − | − | − | − | − |
Citric acid | − | − | + | − | − | − | − | − | − |
D-Galacturonic acid | + | − | + | + | + | + | − | + | + |
β-methyl-D-Glucoside | − | − | + | + | + | + | + | + | + |
D-Glucuronic acid | + | − | − | + | + | + | − | + | + |
α-keto-Glutaric acid | + | − | + | + | + | + | − | − | − |
D-Lactic acid methyl ester | + | + | - | + | + | + | + | + | + |
D-Malic acid | − | + | + | − | − | − | − | − | + |
L-Malic acid | + | + | − | + | + | + | + | + | + |
N-acetyl-Neuraminic acid | + | + | + | − | − | + | − | − | − |
L-Pyroglutamic acid | + | + | − | − | − | + | + | − | − |
Quinic acid | + | + | − | − | − | − | − | − | + |
D-Saccharic acid | − | − | − | + | + | + | + | + | + |
(d) Oxidation of polymer: | |||||||||
Pectin | + | + | + | + | + | + | + | − | + |
Growth in the presence of: | |||||||||
Inosine | + | + | + | + | + | + | + | + | − |
Lithium chloride | + | + | − | − | − | − | + | − | − |
Minocycline | − | − | − | − | − | − | − | + | − |
Potassium tellurite | − | + | − | − | − | + | + | + | − |
Sodium chloride (4%, w/v) | + | + | − | − | − | − | − | − | − |
Sodium bromate | + | + | − | − | − | − | + | − | − |
Sodium formate | − | − | − | − | − | − | + | − | − |
Sodium lactate (1%, w/v) | + | + | − | − | − | − | + | − | − |
Tolerance tests: | |||||||||
Temperature range (°C) | 20–45 | 20–45 | 20–37 | 20–37 | 20–37 | 20–37 | 12–45 | 12–37 | 12–45 |
pH range | 6–9 | 6–9 | 7–9 | 6–8 | 7–8 | 6–8 | 7–9 | 6–8 | 6–9 |
Growth in the presence of NaCl (%, w/v) | 4 | 4 | 1 | 3 | 1 | 1 | 1 | 3 | 1 |
Chemotaxonomy: | |||||||||
Diaminopimelic acid | meso -A 2 pm | meso -A 2 pm | meso -A 2 pm* | meso - and OH-A 2 pm | meso -A 2 pm* | meso - and OH-A 2 pm | meso -A 2 pm* | meso - and OH-A 2 pm | meso -A 2 pm* |
Fatty acids | iso-C16:0 (31.0%), iso-C15:0 (10.6%), iso-C17:0 (11.2%), anteiso-C17:0 (15.1%) | iso-C16:0 (38.5%), iso-C15:0 (7.7%), iso-C17:0 (6.4%), anteiso-C17:0 (11.0%) | iso-C16:0, iso-C15:0, anteiso-C17:0, iso-C17:1ω9c* | iso-C16:0 (11.0%), iso-C15:0 (27.0%), anteiso-C17:0 (10.4%) | iso-C15:0, iso-C17:1ω9c, iso-C17:0, anteiso-C17:0* | iso-C15:0 (23.8%), iso-C17:0 (14.6%), anteiso-C17:0 (12.1%) | iso-C16:0, iso-C15:0, C17:1 cis8* | iso-C15:0 (23.5%), iso-C16:0 (14.8%), 10-methyl C17:0 (14.0%), C17:0 (10.8%) | iso-C16:0, iso-C15:0, iso-C17:0, anteiso-C15:0* |
Menaquinones | MK-9(H4,H6) (24.5, 17.6%) MK-10(H4,H6) (18.4, 16.6%) | MK-9 (H4,H6) (23.1, 10.2%) MK-10(H4,H6) (40.7, 17.6%) | MK-10(H4,H6)* | MK-10(H4,H6) (54.8, 18.1%) | MK-9 (H4) MK-10(H4,H6)* | MK-10(H4,H6,H8) (31.9, 28.9, 19.3%) | MK-10(H4,H6)* | MK-10(H4,H6,H8) (31.7, 24.3, 13.3%) | MK-9 (H4,H6) MK-10(H4,H6)* |
Polar lipids | DPG, PE, PI, GL, 2PL | DPG, PE, PI, GL, 2PL | ND | DPG, PE, PI, GL, 5 L | DPG, PE, PI, GL* | DPG, PE, PI, GL, 2 L | DPG, PE, PI* | DPG, PE, PI, GL, 2PL | DPG, PE, PI, PIM* |
Sugars | gal, glu, man, xyl | gal, glu, man, xyl | ND | gal, glu, man, rham, rib, xyl | gal, glu, man, rib, xyl* | gal, glu, man, rham, rib, xyl | ara, glu, man, rham, rib, xyl* | gal, glu, man, rham, rib, xyl | ara, gal, glu, man, rham, rib, xyl* |
