Figure 5
From: KRAS genotyping by digital PCR combined with melting curve analysis
Fluorescence image of a 2D-array chip and the melting curve measured from changes in the fluorescence intensity of the wells. (A) Fluorescence image of the 2D-array chip at each temperature. During controlled heating, the hybrid of the target and the probe dissociates, and fluorescence is lost. (B) Melting curve of WT KRAS and the G12D mutants derived from the change in fluorescence intensity of a well on a 2D-array chip.
