Figure 6
Imiquimod causes an improvement in respiratory system resistance, tissue damping and tissue hysteresivity. Naïve (control; PBS) or mice infected with Hk x-31 at 105 PFU/mouse were treated once daily via intranasal administration of either imiquimod (50 µg) or vehicle control (PBS) starting 1 day prior to infection up until two days post-infection with analysis occurring at day 3. Mice were anaesthetized and connected to the flexiVent machine and mechanically ventilated at 300 breaths per minute. Lung function measurements were recorded on the fliexiVent machine for respiratory system resistance, tissue damping and tissue hysteresivity. Data are represented as mean ± SEM of n = 4–9 mice. The average of triplicates were taken, where statistical analysis was conducted via a one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons. n.s. denotes not significant, * and ** indicates p < 0.05 and p < 0.01, respectively.
