Figure 6

IFN-γ and TNF-α have different effects on KSHV gene expression in a dose-dependent manner. (A) SLK, iSLK, and iSLK.219 cells were left untreated or induced with Dox (0.2 μg/ml), and at the time of induction, where indicated, IFN-γ or TNF-α (0, 20 or 200 units/ml) was added to the culture. At 2 days post-induction, cell lysates were prepared for Western blot analysis. Temporal expression of KSHV genes was examined: latent, immediate-early (IE), and delayed-early (DE). Arrowhead indicates the specific ORF59 band. β-Actin was included as a loading control. One representative result is shown from 3 independent experiments. The intensity of each specific band was determined using ImageJ software and was normalized to that of the Dox-treated sample. The numbers indicate the relative intensity compared to the Dox-alone control. (B) The means ± SEM of 3 independent experiments are plotted. *P < 0.01 by one-way ANOVA Tukey Method.