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Figure 3

From: Increased susceptibility of airway epithelial cells from ataxia-telangiectasia to S. pneumoniae infection due to oxidative damage and impaired innate immunity

Figure 3

Increased sensitivity in A-T air-liquid interface cultures to S. pneumoniae infection. (A) Immunostaining of individual cell types in differentiated epithelia at ALI. Left panel: goblet cells (MUC5B; green), tight junctions (ZO-1; red); right panel: basal cells (cytokeratin 14; green) and ciliated epithelial cells (Ac α-tubulin; red). (B) Disruption of the respiratory epithelia at ALI by 24 h was observed in A-T but not in controls. (C) Percentage of cell death induced by S. pneumonia infection in control and A-T cells. (D) Transepithelial electrical resistance (TEER) reading performed on control and A-T ALI cultures post-infection. A-T n = 3, healthy controls n = 3. Unpaired, two-tailed Mann-Whitney tests were carried out. p < 0.03 between control and A-T at 48 h. All data were plotted as the mean ± s.d.

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