Figure 2 | Scientific Reports

Figure 2

From: Increase in secreted airway mucins and partial Muc5b STAT6/FoxA2 regulation during Pneumocystis primary infection

Figure 2

Mucin gene-expression and protein quantifications at 45, 60, and 75 days after Pneumocystis co-habitation exposure. Muc5ac mRNA (A), and protein levels (B) and, Muc5b mRNA (C) and protein levels (D) were determined by qPCR and Western blot, respectively. The qPCR and WB Muc5b:Muc5ac ratios are shown in (E and F). Note that scales are different. Each bar represents the mean +/− SD of qRT-PCR determinations calculated using the 2−ΔΔCt method, or the mean +/− SD of the respective lung mucin determination protein levels in 4 rats. Results were normalized by actin and compared with the results of their respective control group. Significance was evaluated by two-way ANOVA and P values were A: **P = 0.0017; B: ****P = 0.0001; C: **P = 0.0082; D: **P = 0.0014 on day 60 and ***P = 0.0002 on day 75. Results are expressed in arbitrary units.

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