Figure 4 | Scientific Reports

Figure 4

From: Increase in secreted airway mucins and partial Muc5b STAT6/FoxA2 regulation during Pneumocystis primary infection

Figure 4

STAT6 pathway activation by Pneumocystis and pharmacological reversal with Kaempferol. Kaempferol was administered by intraperitoneal injection from day 55 to 70 of age. (A) Pneumocystis burden quantified by qPCR amplification of the dhfr gene in DNA from Pneumocystis-negative (control), Pneumocystis-positive without Kaempferol and Pneumocystis-positive with Kaempferol, rat lungs at day 70 of the experiment. (B) Graph plot and (C) Western Blot show that Kaempferol inhibited the Pneumocystis stimulatory effect. Each bar represents the mean ± SD of P-STAT6 protein levels as determined by western blot in lung samples from 4 rats. STAT6 and Actin were evaluated as internal controls. Graph plots were normalized by STAT6 and Actin. Statistical values for comparisons of the means were determined using Mann-Whitney. * indicates P = 0.0286. Pc(−) represents Pneumocystis non-infected rats injected with saline, Pc(+) represents Pneumocystis-infected rats injected with saline, Pc(−)K Pneumocystis non-infected rats injected with Kaempferol and Pc(+)K Pneumocystis-infected rats injected with Kaempferol.

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