Figure 3
One dimensional 13C NMR of Hp and by-products. (A) NMR spectra of 20 mg of samples in D2O were recorded at 313 K on a Bruker 600 MHz spectrometer with DSS as the external reference (0 ppm). Signals were assigned according to Yates et al.23. Sulfation at glucosamine position 6 is denoted 'A6-6S', at position 2 of glucosamine, A2-NS and at position 2 of iduronate, as I2-2S.
