Figure 6
Molecular weight determination of the by-products by gel permeation chromatography against a Hp calibrate. Average molecular weight (Mw) suggested no correlation with purity, however, the polydispersity (Pd = Mw/Mn)) was reduced up to and including the removal of Hp. The loss of sample below 100% in the detected range was due to the resolution range residing between 4 and above 40 kDa (Hp – 99.7%, A – 94.3%, B – 90.3%, C – 94.4%, D – 98.1%). *Mn – number average molecular weight. Average molecular weight (kDa) – Hp, 16.6, A, 30.6, B, 18.7, C, 33.5, D, 26.3. Pd – Hp, 1.2, A, 2.2, B, 2.0, C, 2.1, D, 1.7.
