Figure 2
V. vulnificus PecS is a dimer. (A) Schematic representation of divergently oriented V. vulnificus pecS and pecM genes; VvPecS is expected to bind the intergenic DNA region iSM. (B) Coomassie Blue-stained 15% SDS-PAGE gel showing purified His6-tagged VvPecS (L1) and VvPecS incubated with 0.005% or 0.05% glutaraldehyde (GTA). Oligomeric states are indicated at the right. Mw markers (in kDa) at the left. (C) Size exclusion chromatography. Distribution coefficient as a function of Log10 Molecular weight of markers C, O, and A representing chymotrypsin (25 kDa), ovalbumin (44 kDa) and albumin (66 kDa), respectively. Elution of VvPecS marked with arrow. Uncropped version of gel in panel B shown in Fig. S7.
