Figure 4
DNase I footprint of forward strand of pecS-pecM intergenic DNA. (A) Polyacrylamide sequencing gel: lane L1 – A + G ladder, L2 – undigested iSM fragment (500 nM); L3 – DNase I-digested iSM DNA; L4 to L6 – iSM DNA incubated with 1000 nM, 100 nM and 10 nM VvPecS, respectively, followed by DNase I digestion. (B) Densitometric traces of VvPecS protection of iSM (500 nM) DNA upon incubation with 0 nM (green), 500 nM (orange), 1,000 nM (blue) and 5,000 nM (red) VvPecS. C. Sequence of iSM intergenic sequence. The start codons of pecS and pecM are marked by solid black arrows. The pseudo-palindromic sequences are represented by orange bolded letters and protection by 1,000 nM PecS is underlined in blue. Reactions were performed under stoichiometric conditions ([DNA] ≫ Kd). Data are representative of three experiments. Uncropped version of gel in panel A shown in Fig. S9.
