Figure 6
In vivo lacZ reporter gene expression assay. E. coli T7 Express was transformed with pecS expression construct and/or reporter construct: (pr0) – empty pACYC184 and pET28b; (prSZ) – lacZ under control of pecS promoter and empty pET28b; (prSZ + pecS) – lacZ under control of pecS promoter and pecS expression construct. Black bars represent uninduced cultures (no IPTG) and grey bars induced cultures (80 μM IPTG to induce pecS expression). Where indicated, xanthine was included at 10 mM concentration. β-galactosidase activity is represented in Miller units. Error bars represent the standard deviation from three replicates. Asterisks represent statistically significant difference based on two-tailed Student’s t-test (*p < 0.05 and **p < 0.005).
