Figure 7
VvPecS represses nod promoter activity in vivo. In vivo lacZ reporter gene assay in which E. coli T7 Express was transformed with pecS expression construct and/or reporter construct. β-galactosidase levels (in Miller units) in uninduced E. coli expression host harboring gene constructs: (pr0) – empty pACYC184 and pET28b; (prSZ) – lacZ under control of pecS promoter and empty pET28b; (prNZ) – lacZ under control of nod promoter and empty pET28b; (prNZ + pecS) – lacZ under control of nod promoter and pecS expression construct. (A) Comparison between prSZ and prNZ promoter activities in the absence of VvPecS (asterisks indicate p < 0.005). (B) Activity of prNZ in absence or presence of NONOate (25 μM). (C) Effects of VvPecS and xanthine on activity of prNZ. Asterisks indicate p < 0.005 compared to (prNZ) cells based on two-tailed Student’s t-test. Error bars represents standard deviation from three replicates.
