Figure 2
Increased expression levels of TRPV1, CAMKII, and phosphorylated ERK in the ipsilateral dorsal horn induced by chronic constriction injury. (A) The loose ligation of the sciatic nerve significantly increased TRPV1 and CAMKII expression in the ipsilateral spinal dorsal horn 7 days after CCI. (B) Bar chart showing the protein content relative to the sham group. The levels of TRPV1 and CAMKII in the ipsilateral spinal dorsal horn of the CCI group were significantly higher than those in the sham group (P = 0.031 for TRPV1; P = 0.018 for CAMKII). (C) Loose ligation of the sciatic nerve significantly increased ERK phosphorylation in the ipsilateral spinal dorsal horn. (D,E) Bar charts depicting the protein content relative to that in the sham group. A significantly higher level of phosphorylated ERK2 was observed in the ipsilateral spinal dorsal horn of the CCI group than in the ipsilateral spinal dorsal horn of the sham group (P = 0.023). GAPDH or tubulin served as a loading control and was run on the same blot. *P < 0.05 compared with the sham group (ANOVA, n = 4–5 rats per group). Error bars represent the SEM. CCI: chronic constriction injury; Contra: contralateral to CCI.
