Figure 3
The intrathecal injection of the TRPV1 siRNA in vivo attenuated the mechanical and thermal hyperalgesia induced by chronic constriction injury. (A) The administration of the TRPV1 siRNA (5 μg/15 μl) once daily for two days significantly increased the paw withdrawal threshold to mechanical stimuli on days 1 to 4 post-transfection compared to the CCI + PEI control group or to the pre-injection baseline. (B) The TRPV1 siRNA significantly increased the paw withdrawal latency in response to thermal stimuli on days 1 to 4 post-transfection compared to results in the CCI + PEI control group or the pre-injection baseline. **P < 0.01 and *P < 0.05 compared with the PEI group results; ##P < 0.01 and #P < 0.05 compared with the pre-injection baseline results (ANOVA, n = 5–6 rats per group). Error bars represent the SEM. PEI: polyethylenimine; CCI: chronic constriction injury.
