Figure 1
The beating rate, cell viability, and quantity of intracellular TAGE in cardiomyocytes treated with GA. (a,b) Beating rates were assessed in three independent experiments. One experiment was performed to count the beating rates of cardiomyocytes in 4 circular areas (diameter of 2 mm) in 35-mm dishes in order to calculate the average. Data are shown as means ± S.D. (N = 3). P-values were based on Dunnett’s test. **p < 0.01 vs. the control. (c,d) Cell viability was assessed by the WST-8 assay. This assay was performed in three independent experiments. One experiment was performed using 4 wells to calculate the average. Data are shown as means ± S.D. (N = 3). P-values were based on Dunnett’s test. **p < 0.01 vs. control. (e,f) Intracellular TAGE were analyzed with a slot blot (SB) analysis. Cell lysates (2.0 μg of protein/lane) were blotted onto a polyvinylidene difluoride (PVDF) membrane. The amount of TAGE was calculated based on a calibration curve for GA-derived AGE-BSA (TAGE-BSA). A SB analysis was performed in three independent experiments. Data are shown as means ± S.D. (N = 3). P-values were based on Dunnett’s test. **p < 0.01 vs. the control.
