Table 5 Palmitoylation and myristoylation of IDAMPs improves S. aureus ATCC 6538-bactericidal activity of HR1-18, HR1-11 and its Arg-mutants.

From: Cationic Intrinsically Disordered Antimicrobial Peptides (CIDAMPs) Represent a New Paradigm of Innate Defense with a Potential for Novel Anti-Infectives

AA-sequence

Name

n

LD100 (µg/mL)

LD90 (µg/mL)

Condition

Pal-GRHGSGLGHSSSHGQHGSGSGR

Pal-HR 1-18, Pal-HRNR2656–2677

1

>300

>300

pH7.3, 1% TSB

Pal-GRHGSGLGHSSSHGQHGSGSGR

Pal-HR 1–18, Pal-HRNR2656–2677

2

37.5

1.18

pH5.5, 0.25% glucose

Myr-GRHGSGLGHSSSHGQHGSGSGR

Myr-HR 1-18, MyrHRNR2656–2677

1

75

9.38

pH5.5, 0.25% glucose

Myr-GRHGSGLGHSSSHGQHGSGSGR

Myr-HR 1-18, MyrHRNR2656–2677

1

75

18.75

pH7.3, 1% TSB

Myr-GRRGSGLGRSSSRGQRGSGSGR

Myr-HR 1-18-HR

1

75

18.75

pH7.3, 1% TSB

Pal-GRRGSGLGRSSSRGQRGSGSGR

Pal-HR 1-18-HR

2

4.7

0.3

pH5.5, 0.25% glucose

Pal-GRRGSGLGRSSSRGQRGSGSGR

Pal-HR 1-18-HR

1

9.38

1.18

pH7.3, 1% TSB

Pal-GRRGSGLGRSSSR

Pal-HR 1-18-3H3R-GR13

2

37.5

2.35

pH5.5, 0.25% glucose

Pal-HGSRSGQSSRGERHGSSSGSSSH

Pal-HR 1-11, HRNR2606–2628

1

>300

2.35

pH5.5, 0.25% glucose

Pal-RGSRSGQSSRGERRGSSSGSSSR

Pal-HR-1-11-3H3R

2

300

0.59

pH5.5, 0.25% glucose

Pal-RGSRSGQSSRGERR

Pal-HR-1-11-2H2R-14

1

18.75–75/>300*

0.59/>300*

pH5.5, 0.25% glucose

  1. *Antimicrobial paradox16 (increased bacterial growth with increasing CIDAMP-concentrations, see (Fig. 3). Data shown are representatives. n: number of experiments.
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