Table 3 Distribution of vsRNA abundance along TYLCV genome.

From: Integrated single-base resolution maps of transcriptome, sRNAome and methylome of Tomato yellow leaf curl virus (TYLCV) in tomato

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Region

Start

End

Size

Number of vsRNA per nucleotide

 

Agrob-TYLCV (dpi)

Bemisia-TYLCV (dpi)

7

14

21

7

14

21

IR

1

313

313

30

207

232

6

114

192

 

V2

314

472

159

76

739

615

5

413

543

 

V2/V1

473

673

201

343

1356

1336

72

1059

1430

OL1

V1-C3

674

1391

718

119

569

481

75

476

587

 

C1/C2/C3

1392

1799

408

303

1627

1708

67

1317

2155

OL2

C1- 3′end

1800

2336

537

109

534

488

23

361

473

 

C1/C4

2337

2628

292

255

1628

1741

55

1062

1616

OL3

C1- 5′end

2629

2781

150

19

128

119

4

78

126

 

Genome

1

2781

2781

146

832

818

31

616

893

 
  1. Tomato plants were infected by either agroinoculation (Agrob-TYLCV) or by B. tabaci (Bemisia-TYLCV) and data from different time points during the systemic infection are shown (7, 14 and 21 dpi). The values represent the number of vsRNA (20–25 nt) per nucleotide at distinct genomic regions according to the position of the viral 5′nucleotide. The regions are identified by the viral genes they encompass, their size (nt) and their coordinates according to the TYLCV genome map are indicated at the top. The three genomic regions that showed the maximum number of vsRNA per nucleotide (OL1, OL2 and OL3) are marked in bold. Data comes from the same biological replicates showed in Fig. 4A,B.