Figure 6 | Scientific Reports

Figure 6

From: Genotoxic stress increases cytoplasmic mitochondrial DNA editing by human APOBEC3 mutator enzymes at a single cell level

Figure 6

Abundant A3-edited cymtDNA in single cells. (a) Number of hypo- and hypermutated mtDNA sequences obtained using a PCR denaturation temperature of 95 °C; anumber of analyzed sequences; bnumber of hypomutated sequences; cproportion of hypoedited sequences per cell; dmean CG-> TA mutation frequencies for hypoedited sequences, enumber (#) of hyperedited mtDNA sequences identified; fnumber (#) of C-> T or G-> A transitions per mtDNA sequence. (b) A selection of hypermutated MT-COI sequences in presence or absence of 100 μM actinomycin D in P2 cells and in purified CD4+ from patient D2. Ref corresponds to the MT-COI reference sequence. Only differences are shown. To the right are the number mutations per sequence edited.

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