Figure 8

Syntaxin 6 is upregulated in metastatic RCC cell lines and regulates survival of metastatic papillary RCC cells. (A) Syntaxin 6 protein expression was measured by Western blotting in ccRCC (786-O and Caki-1) and pRCC (Caki-2 and ACHN) cell lines. Membrane was also probed for tubulin to assess protein loading. (B) ACHN cells were transfected with siRNA for syntaxin 6 as described in methods and syntaxin 6 knock-down was confirmed by real-time PCR. Relative syntaxin 6 mRNA level compared to GAPDH in control cells and syntaxin 6 knock-down is presented. (C) ACHN cells were transfected with siRNA for syntaxin 6 and cell viability was measured after 72 hrs in MTT assay. *p < 0.001.