Figure 2
From single iMSD traces to whole-cell-population analysis. (A) iMSD curves from 107 cell acquisitions of proinsulin-EGFP expressing INS-1E cells with the average highlighted in bold green. (B) Normalized relative distributions of Dm, α, and size parameters. (C) Protein expression level analysis. Each point in the plots associates, for each acquisition, the obtained value of Dm (top), α (middle), and size (low panel) with the protein expression level (expressed in fluorescence counts). Solid green lines represent the average values calculated for each parameter, plus/minus standard deviation (dashed green lines). Solid red lines are calculated by averaging data points with a floating window corresponding to 8 experimental points. Dashed green lines represent standard deviation of all experimental data points. (D) Structural and dynamic properties of ISGs labelled with proinsulin-EGFP represented as a scatter plot in which the three values of α, Dm and size of each acquisition are organized in a 3-D parametric space. Darkness of the color represents the fluorescence expression level. The 3D-plot projections of data points show the confidence ellipsoids of average values and SD.
