Figure 4

eEF3 depletion causes moderate redistribution of the ribosome density along mRNAs. The graphs show distributions of polarity scores for individual ORFs (a,b, two experimental replicates), and average ribosome density in the vicinity of start and stop codons (c,d, two experimental replicates). The expression of eEF3 was either repressed by the addition of 0.5 mM methionine (red trace) or unrepressed (black trace, no methionine). The locations of start and stop codons are highlighted with green and blue backdrops, and the corresponding A-, P- and E-site positions of initiating and terminating ribosomes are indicated with letters under the x-axis.