Figure 2
From: Direct imaging of the recruitment and phosphorylation of S6K1 in the mTORC1 pathway in living cells
Live cell interaction between S6K1 and raptor using FRET-FLIM in HEK293 cells. (a) Confocal image of EGFP-S6K1. (b,c) FLIM of EGFP-S6K1 with corresponding lifetime distribution histogram showing lifetime (τ). (d–g) Confocal images of EGFP-S6K1 with mCherry-raptor co-expression with FLIM and lifetime distribution histogram. (h) Schematic showing summary of interaction. (i) Graph showing cytoplasmic against whole cell lifetimes between EGFP-S6K1 and mCherry-raptor, selected by selecting subcellular regions and obtaining the mode lifetime in SPCImage V6.0. Data representative of three independent experiments with errors representative of standard deviation.
