Figure 6
p38α MAPK silencing in primary hepatocytes increases binucleation through blockade of MNK1 phosphorylation. (A) Representative images of isolated hepatocytes treated with scramble, p38α MAPK siRNA and Mnk1 siRNA (scale bars = 50 µm). Control for p38α MAPK and Mnk1 silencing by siRNA in isolated hepatocytes is also shown. (B) Quantification of the binucleation rate (%) (binucleated hepatocytes/total hepatocytes) in scramble-treated, p38α MAPK siRNA-treated and Mnk1 siRNA-treated primary culture hepatocytes. At least 20 fields per condition were examined. Data are shown as mean and SD. **P < 0.01. (C) Western blot of isolated hepatocytes scramble-treated, p38α MAPK siRNA-treated and Mnk1 siRNA-treated for p-p38, p38α, p-MNK1, MNK1 and p21. α-tubulin was used as loading control. The number of samples per group was 4 to 8.
