Figure 2
From: Global Implications of Local Unfolding Phenomena, Probed by Cysteine Reactivity in Human Frataxin
Equilibrium unfolding experiments of FXN Cys-mutants. Urea-induced equilibrium unfolding followed by (A) CD at 220 nm and (B) tryptophan fluorescence at 335 nm (ex. 295 nm). (C) Thermal unfolding followed by CD at 220 nm. Solid lines represent the nonlinear regression fit of the two-state model to the data.
