Figure 4
From: Global Implications of Local Unfolding Phenomena, Probed by Cysteine Reactivity in Human Frataxin
Cysteine modification experiments. The reactivity of FXN Cys-mutants and Cys-peptides was determined for DTNB (A–C) and DTDPy (D–F). (A,D) show the time traces of the reactions with 1 mM DTNB (A) or DTDPy (D). Similar curves were obtained at different probe concentrations. A monoexponential function of time was fitted to the data in order to find klabel for each mutant (B and E). The reaction intrinsic constant, kmod, was obtained from a similar fitting to the Cys-peptides’ curves (C and F). We obtained the concentration-independent second-order kinetic rate constants from the linear regression of pseudo-first order constants found each probe concentration (B, C, E and F).
