Table 2 Local (\({\rm{C}}\rightleftharpoons {\rm{O}}\)) and global (\({\rm{N}}\rightleftharpoons {\rm{U}}\)) unfolding equilibrium constants.
From: Global Implications of Local Unfolding Phenomena, Probed by Cysteine Reactivity in Human Frataxin
FXN L198C | FXN L200C | FXN L203C | ||
|---|---|---|---|---|
\({\rm{C}}{\rm{\rightleftharpoons }}{\rm{O}}\) equilibrium DTNB | \({\rm{\Delta }}{{G}}_{{\rm{C}}\rightleftharpoons {\rm{O}}}^{{\rm{o}}}\) | 5.01 ± 0.03 | 3.31 ± 0.02 | 5.60 ± 0.01 |
\({{K}}_{{\rm{C}}\rightleftharpoons {\rm{O}}}\) | 0.00021 ± 0.00001 | 0.0037 ± 0.0001 | 0.000078 ± 0.000002 | |
fC/fO† | 4 704 ± 207 | 267 ± 10 | 12 760 ± 303 | |
\({\rm{C}}{\rm{\rightleftharpoons }}{\rm{O}}\) equilibrium DTDPy | \({\rm{\Delta }}{{G}}_{{\rm{C}}\rightleftharpoons {\rm{O}}}^{{\rm{o}}}\) | 4.39 ± 0.03 | 2.49 ± 0.03 | 4.79 ± 0.03 |
\({{K}}_{{\rm{C}}\rightleftharpoons {\rm{O}}}\) | 0.00061 ± 0.00003 | 0.0149 ± 0.0007 | 0.000308 ± 0.000014 | |
fC/fO† | 1652 ± 84 | 67 ± 3 | 3252 ± 144 | |
\({\rm{N}}{\rm{\rightleftharpoons }}{\rm{U}}\) equilibrium Urea, CD | \({\rm{\Delta }}{{G}}_{{\rm{N}}\rightleftharpoons {\rm{U}}}^{{\rm{o}}}\) | 8.1 ± 0.5 | 7.2 ± 0.5 | 9.8 ± 0.6 |
\({{K}}_{{\rm{N}}\rightleftharpoons {\rm{U}}}\)* | 0.0000012 ± 0.0000004 | 0.000005 ± 0.000001 | 0.00000006 ± 0.00000003 | |
fN/fU† | 859 672 | 204 283 | 15 677 417 | |
