Figure 8

Histopathology of the liver after IRE ablation with H&E stain. Three hours (3 h) after IRE the lobular architecture is preserved although with visible hemorrhagic, congestion, and edema(a–d). After three (3d) and seven days (7d), two zones can be distinguished: one necrotic (nz) and the other with signs of cellular regeneration (rz) (e,i). The hepatocytes of the electroporated area appear irregular and with disfigurement of the membranes, apparently intact nuclei although some of them with karyolysis (arrows, (c,d and h)). The centrilobular vein (cv) and the hepatocyte trabeculae are distorted with loss of hepatocytes (arrows) and endothelial cells (b,f and j). The reparative area show: new bile ducts and vessels (asterisks, (g,k)); mesenchymal spindle cells infiltrating the necrotic area (arrowheads, (h)) and regenerative nodules of hepatocytes with acinar arrangement and mitotic figures (arrows, (l)).