Figure 9

Electron micrograph of pig liver after IRE. Portion of a liver lobule showing plates of hepatocytes damaged but preserving its structure (b–d) 2,000X. Under TEM, the hepatocytes show lysis of the plasma membrane with the release of organelles, dilatation of the granular endoplasmic reticulum and mitochondrial alterations but the nuclear envelope is preserved (arrowhead, f,g). After 7 days (7d), phagocytic cells are frequent, phagocytizing cell debris (h). SEM of large blood vessel, after 3 hours (3 h) and 3 days (3d), illustrating the lack of endothelial cells and their substitution by platelets (arrows) (j,k) 2,000X. After 7 days, endothelial cells are distinguished in some vessels (l). Controls were taken in healthy tissue (a,e and i). Scale bar (a–d,i–l) 10 μm.