Figure 2
Mapping and cloning of ESC1. (a) Map-based cloning of ESC1. Five SSLP markers on chromosome 2 are indicated on the top. The physical distance of each markers and the numbers of informative recombinants from 310 F2 plants are shown below. Note that the mutation site was mapped to a 90-kb region at the end of chromosome 2. (b) Schematic diagram of At2g48060 gene structure. Exons and untranslated regions of exons are indicated by black and gray boxes, respectively. The lines between the boxes represent introns. The mutation site of esc1-1 in the 18th exon was indicated by an arrow head. Note that the T-DNA insertion of esc1-2 (Salk_003005) is in the 6th intron. Bar, 500 bp. (c) Pathogenic responses of Col-0, esc1-1, esc1-2, and their F1 progeny at 14 days (upper) and 49 days (bottom) after infection with mock or CMV-2aTΔ2b. F1 plants were from the cross of esc1-1 (female) and esc1-2 (male) and a reciprocal cross as indicated. Bars, 3 cm. Accumulation of CMV-2aTΔ2b genomic/subgenomic RNAs (d) and viral CP (e) in the systemically infected leaves of the plant samples shown in (c) at 14 dpi. (f) Symptoms of Col-0, esc1-1, esc1-3 and esc1-4 inoculated with mock or CMV-2aTΔ2b at 14 dpi (upper) or 49 dpi (bottom). Bars, 3 cm. Accumulation of CMV-2aTΔ2b RNA (g) and viral CP (h) in the systemically infected leaves of the plant samples shown in (f) at 14 dpi. Methylene blue-stained rRNA were used as loading controls for total RNAs. In the d and g, the bottom values represent relative accumulation (RA) of the CMV RNA3. In the e and h, the bottom values represent RA of CMV CP. All the band intensities were evaluated by ImageJ and the RA values of Col-0 were set as 1. The RbcL was stained by Coomassie blue as protein loading controls.
