Figure 3
The A. thaliana mutant esc1-2 and esc1-3 exhibited enhanced susceptibility to wild type CMV. (a) Symptoms of A. thaliana Col-0, esc1-2 and esc1-3 inoculated with mock or wild type CMV at 5 dpi and 10 dpi. Col-0, esc1-2 and esc1-3 were inoculated with mock buffer or purified CMV virions (20 μg/mL). Bars, 1 cm. (b,c) Western blotting analysis to detect accumulations of CMV CP in inoculated leaves at 4 dpi (b) or systemically infected leaves at 5 dpi (c). Bottom values represent relative accumulations (RA) of CMV CP. The RbcL was stained as loading controls. Three independent repeats were indicated as 1, 2, and 3. (d,e) Detection of CMV RNA accumulation in inoculated leaves at 4 dpi (d) and systemic leavesat 5 dpi (e) by qRT-PCR. Actin2 served as the internal control. The error bars represent SD from three independent experiments and letters above bars indicate statistically significant differences. According to the Duncan’s multiple range test, P > 0.05 in (d) and P < 0.01 in (e).
