Figure 5
Tissue specific expression of AtPiezo. Expression analysis of AtPiezoPro::GUS in different plant tissues. GUS activity was detected in root (a), petiole (b), flower petals (d) and silique stalk (e). Cross section of petioles (c) showed that GUS was highly expressed in the vasculature of petioles. Bars, 1 cm (a,b), 0.5 mm (c–e). (f) Two independent transgenic Col-0 plant lines harboring AtPiezoPro-drived a GUS transgene were stained to show tissue specific expressions of GUS after mock treatment (left) and CMV-2aTΔ2b infection (middle) at 2 dpi. Detached leaves from the transgenic plants were shown in the right panels. Note the strong expression of GUS in the petioles. r, rosette leaves; h, hypocotyls. Bars, 1 cm. (g) Quantitative real-time PCR to detect the AtPiezo mRNA expression level in petioles of inoculated leaves by mock or CMV-2aTΔ2b at 2 dpi. Data points are the mean value of three independent experiments and error bars represent the standard deviation (s. d.). Asterisks indicate statistically signifcant difference according to Student’s t-test, **P-value < 0.01.
