Figure 9

Structural confirmation of lipid annotations by on-tissue tandem mass spectrometry (MS/MS) in negative- and positive-ion mode, using high-energy collisional dissociation (HCD). (A) On-tissue HCD-MS/MS (averaged) spectrum of [PG(34:1) − H]− m/z 747.51966 ± 0.4. (B) On-tissue HCD-MS/MS (averaged) spectrum of [LPC(16:0) + H]+ m/z 496.34007 ± 0.4.