Figure 1
From: Enriched environment alleviates stress-induced dry-eye through the BDNF axis
Stress decreases tear secretion. (a) Measurement of tear secretion volume using cotton thread test. The sample image shows 7. 9 mm length; 7 mm by a ruler (middle) and 0.9 mm by a scale (right). (b) Model of stress loading. (c) Tear secretion significantly decreased in response to restraint and blowing (n = 5; **P < 0.01 by one-way ANOVA with post-hoc Tukey’s test). (d) Within 15 days, the groups with 1 or 2 hours of stress showed recovery regardless of stress loading, while the group with 4 hours of stress maintained lowered tear secretion (n = 6–7; **P < 0.01, *P < 0.05 by one-way ANOVA with post-hoc Tukey’s test). (e) Four hours of stress was necessary to maintain reduced tear secretion (n = 6; **P < 0.01 by Student’s t-test). (f) Longer duration of stress loading resulted in a longer durations of maintenance and time required for recovery (n = 4–5).
