Figure 5
From: Enriched environment alleviates stress-induced dry-eye through the BDNF axis
Bdnf isoforms 4 and 6 are necessary for enriched environment-related alleviation of stress-induced decreased tear secretion. (a) Model of Actin-tTS::BdnftetO/tetO mice (Actin). (b) Bdnf expression in the hippocampus (HC), hypothalamus (HT), and lacrimal gland (LG). Actin-tTS::BdnftetO/tetO mice showed no expression of exons 4 and 6 in the brain. Total Bdnf expression decreased significantly in the LG (n = 4–6; **P < 0.01, *P < 0.05, Student’s t-test). (c) Basal tear secretion in Actin-tTS::BdnftetO/tetO mice was similar to that in the control mice (n = 5–8). (d) Actin-tTS::BdnftetO/tetO mice did not maintain tear secretion under stress loading regardless of the presence of an enriched environment (EE) (n = 6; **P < 0.01, Student’s t-test).
