Figure 2

Effects of treatment by CeO2 NP on the viability and apoptosis of HSFs. (A) HSFs were treated with different concentrations of CeO2 NP for 24, 48 and 96 h, respectively. Then, HSF cell viability was evaluated by the CCK-8 assay. (B) Flow cytometric profiles of Annexin V-FITC/PI -stained apoptotic cells treated with CeO2 NP. (C) Fluorescence images using Calcein-AM staining. The scale bars in these images represent 50 µm.