Figure 7

Effects of spicatoside A on cell viability. (A) Huh7.5 or (B) A549 cells were treated with varying concentrations of spicatoside A (0, 1, 2, 5 and 10 µg/ml). Cells were re-treated with spicatoside A every 3 d after the initial treatment. Huh7.5 and A549 cell viability was determined at 4 and 14 d, respectively, after treatment using the CellTiter-Glo Luminescent Cell Viability Assay. Relative luciferase activity was calculated by reference to RLU of the 0 µg/ml LPE treatment group and defined as 100%. P-values were determined by unpaired two-tailed Student’s t-tests (significant at **P < 0.01; ***P < 0.001). The data shown here represent five independent experiments.