Figure 5
NMDAR mediated nNOS activation is prevented by LOPC at 15 days. (A) Calcium accumulation in the thalamus is not affected by LOPC at all pixel intensities as shown by cumulative tresholding analysis (scalebar = 400 μm). (B) The thalamic expression NR1, N2B and nNOS do not change in time or with treatment as assessed by WB. At 15 days, WB analysis show that the expression of synaptic marker PSD-95 is decreased by 50% in LOPC while Synapsin 1 expression is unchanged. (C) Co-immunoprecipitation of N2B and PSD-95 shows decreased interaction in LOPC samples between NMDAR and PSD-95 at 15 days (-Ab: beads not coated incubated in lysate; -Lysate: beads coated incubated in PBS). Images cropped from Supp. Fig. 3. Results are shown as the mean ± SD. *p < 0.05, Mann Whitney U test.
