Figure 3

Transgene expression after intrastriatal injections of the HiRet and NeuRet vectors in macaque monkeys. Intrastriatal injections of HiRet (2.5 × 10¹⁰ genome copies/ml) and NeuRet (2.5 × 10¹⁰ and 7.5 × 10¹⁰ genome copies/ml) pseudotyped lentiviral vectors were made in macaque monkeys, and histological analyses were performed in the brains fixed at four weeks postinjection. (a) RFP immunostaining in the substantia nigra pars compacta (SNc), centromedian nucleus (CM), and supplementary motor area (SMA) of the ipsilateral and contralateral hemispheres for the HiRet (top), lower-titer NeuRet (middle), and higher-titer NeuRet (bottom) vectors. cp, cerebral peduncle; MD, mediodorsal nucleus; SNr, substantia nigra pars reticulate; III, layer III; V, layer V. Scale bars, 1 mm for SNc and CM, 500 μm for SMA. (b) Number of RFP-positive neurons in the SNc and the centromedian-parafascicular complex (CM-Pf). Eleven or seven sections were used for cell counts in the SNc or CM-Pf, respectively. (c) Density of RFP-positive neurons in the SMA of the ipsilateral and contralateral hemispheres. Ten sections were analyzed. Each symbol (open or filled circle, triangle, or square) indicates data for each animal, and the same symbol represents data for the same animal. Averaged data (n = 2) are denoted by open (for the HiRet vector), filled (for the NeuRet vector with lower titer), and gray (for the NeuRet vector with higher titer) bars.