Figure 4
From: Distinct isoforms of Nrf1 diversely regulate different subsets of its cognate target genes
Nrf1-related genes in interaction networks and their expression regulated by different Nrf1 isoforms. (A) Two interaction networks for Nrf1 were established on the base of the BioGRID database and STRING database. The interactor nodes showing up-regulation or down-regulation were marked in red or green, respectively. Such genes were marked with gradient colours from green to red according to the Log2 (fold changes), but gray indicates that a gene was not expressed. (B) The expression comparison of Nrf1, Nrf2 and Nrf3, Bach1 and Bach2, together with small Maf family members. (C–E) Expression of Keap1 (Kelch-like ECH-associated protein 1) at its mRNA levels (C) and protein levels (D,E) was determined in the presence or absence of Nrf1α and/or Nrf2. The expression of Nrf1α and Nrf2 at protein levels was shown in different gels at the same amount of samples (the left side of D). Then the relative expression of Keap1 at its protein levels (E) was calculated by the mean fold change relative to control based on the grayscale values of three independent experiments (the right side of D). The error bars indicate mean ± SD (n = 3), with significant decreases (*p < 0.05, **p < 0.01) being calculated relative to the corresponding control values.
