Figure 6

Fluorescence microscopic images of staining annexin V-FITC/PI for apoptotic morphology of HT-29 cells treated with five MFGMs (100 μg/mL) for 72 h. Normal HT-29 cells were used as control cells. Cells stained with V-FITC turn green and indicate they are in the earlier apoptotic phase. While cells stained with both V-FITC (which stains the cell membrane green) and PI (which stains the nuclei red) turn orange, this indicates the cells are at later stages of apoptotic phase. Cells stained with red are only labelled with PI and indicate the dead cells. The magnification is ×200.