Table 1 Primers used in this study.
Primer name | sequences (5′ to 3′) | Primer use |
|---|---|---|
AP24-F | CTCATATGGCCACTATCGAGGTCC | Vector construction |
AP24-R | GGTCTAGATTAACCATTAGGAC | |
Gluc-F | GGATCACCCAAAGTTGATATTATATTTGG | Vector construction |
Gluc-R | GCTCAATCGATAGGTGTTTGC | |
T7UTR-F | GGAGACCACAACGGTTTCC | Vector construction |
T7UTR-R | GTTATCGATTGCATATGTATATCTCC | |
Fw1 | GTATCTGGGGAATAAGCATCGG | PCR analysis |
Rv1 | CGATGACGCCAACTACCTCTG | |
Fw2 | CATACTTGAAGCTAGACAGG | PCR analysis |
Rv2 | CTCTACCACTGAGCTAATAGCC | |
qPCR1-F | CTTCTCACCGAGGCTCCACT | qPCR |
qPCR1-R | TGGAACCGTATGCGTCACTC | |
qPCR2-F | TTCACATCCAGGGTGGTCAG | qPCR |
qPCR2-R | CACGGACCGAGTCCATTGTA | |
qPCR3-F | GCTGCTGGCATCTTTTTGCT | qPCR |
qPCR3-R | TTCATCGATGTGCGAGCCTA | |
qPCR4-F | CACGGACCAAGGAGTCTGACAT | qPCR |
qPCR4-R | TCCCACCAATCAGCTTCCTTAC |
