Figure 1 | Scientific Reports

Figure 1

From: Absence of Global Stress Regulation in Escherichia coli Promotes Pathoadaptation and Novel c-di-GMP-dependent Metabolic Capability

Figure 1

RpoS deficiency in NMEC. (a) Low c-di-GMP levels of NMEC strains (RS218, IHE3034F and IHE3034) compared to those of UPEC strains (536 and UTI89) due to RpoS deactivation. When RpoS activity was restored, the c-di-GMP levels of IHE3034 reached the levels of the K-12 MG1655 strain. (Data are represented as the mean +/− SD). (b) Schematic comparison between NMEC and UPEC. NMEC is schematically present in blue, while a UPEC cell that has the same K1 serotype (such as UTI89) of NMEC is shown in red. The common features are present in the intersection. (c) Immunoblotting against RpoS of different E. coli groups. Strain RH90 (MC4100∆rpoS) was used as a negative control. H-NS levels were used as a loading control. (d) Rugose colony formation by IHE3034 derivative strains with restored RpoS. Only the strains having pMMkatF showed rugose colony formation at 22 °C.

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